中华医学杂志（英文版）2012，Vol.125Issue(19)：3458-3464,7.DOI:10.3760/cma.j.issn.0366-6999.2012.19.018
Molecular epidemiology of Mycobacterium tuberculosis in Gansu province of China
Molecular epidemiology of Mycobacterium tuberculosis in Gansu province of China
摘要
Abstract
Background Mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) and Beijing family typing based on detecting the deletion of RD105 sequence are two common genotyping methods used to study the molecular epidemiologic characteristics of Mycobacterium (M.) tuberculosis.We collected 218 strains of M.tuberculosis between 2004 and 2006 in the Linxia Hui Autonomous Prefecture of Gansu province in Northwest China.Methods MIRU-VNTR analysis and Beijing family typing based on detecting the deletion of RD105 sequence were used to type the 218 strains,and their typing power was evaluated to look for practical and efficient genotyping methods suitable for the region.Results The MIRU typing yielded 115 distinct genotypes,including 98 unique isolates and 17 different clusters containing 120 isolates (55.05％); the cluster rate was 47.25％.By detecting the deletion of RD105 sequence,188 of 218 (86.23％) isolates belonged to Beijing family.Combination of Beijing family typing and MIRU typing yielded 118 distinct patterns,including 101 unique isolates and 17 clusters containing 117 isolates (54.13％).The largest cluster contained 58 strains with MIRU genotype of 223325173533 which contained 50 strains belonging to Beijing family and 8 strains belonging to non-Beijing family.Conclusions The Beijing family strains occupied a large proportion and the Beijing family MIRU genotype 223325173533 is a dominant strain in Linxia of Gansu.Combining detecting the deletion of RD105 and MIRU typing together provides a simple,fast,and effective method which is low in cost and might be practical and suitable for M.tuberculosis genotyping in China.关键词
Mycobacterium tuberculosis/ mycobacterial interspersed repetitive unit genotyping/ Beijing family/epidemiologyKey words
Mycobacterium tuberculosis/ mycobacterial interspersed repetitive unit genotyping/ Beijing family/epidemiology引用本文复制引用
TIAN Li-li,ZHU Bing-dong,SI Hong-yan,MU Tao-jun,FAN Wen-bing,WANG Jing,JIANG Wei-min,LI Qing,YANG Biao,ZHANG Ying..Molecular epidemiology of Mycobacterium tuberculosis in Gansu province of China[J].中华医学杂志（英文版）,2012,125(19):3458-3464,7.基金项目
This work was supported by grants from the Fundamental Research Funds for the Central Universities (No.lzujbky-2010-k07) and the Program for New Century Excellent Talents in University (No.NCET-07-0403). （No.lzujbky-2010-k07）
