肿瘤防治研究2012，Vol.39Issue(8)：947-949,3.DOI:10.3971/j.issn.1000-8578.2012.08.016
人肝细胞肝癌和癌旁正常组织microRNA表达差异的分析
microRNA Expression Profiles in Hepatocellular Corcionma and Adjacent Nontumorous Tissues
肖海静 1王观宇 2董庆华3
作者信息
- 1. 430020 江汉区常青街社区卫生服务中心
- 2. 浙江大学医学院附属邵逸夫医院普外科
- 3. 浙江大学医学院附属邵逸夫医院中心实验室
- 折叠
摘要
Abstract
Objective To investigate the microRNA expression profiles in hepatocellular carcinoma(HCC) and adjacent non-tumorous tissues. Methods The relative expression of 474 miRNA genes was determined using a human miRNA microarray. Target and reference samples were co-hybridized to a microar-ray containing whole human mature and precursor miRNA sequences. Results Compared with the corresponding adjacent non-tumorous tissue,one hundred and sixteen miRNAs including miR-181 ,miR-21 and let-7e in the array were found to be highly expressed and 97 miRNA including miR-199, miR-451 and miR-122 showed low levels of expression in hepatocelluar carcionoa tissue. Conclusion This study provides genomewide survey of human miRNA transcripts in HCC. It may help clarify the molecular mechanisms involved in the progression of HCC,potentially serving as a diagnostic tool of HCC.关键词
肝细胞肝癌/微阵列芯片技术/microRNAKey words
Hepatocellular cacinoma/Microarray/microRNA分类
医药卫生引用本文复制引用
肖海静,王观宇,董庆华..人肝细胞肝癌和癌旁正常组织microRNA表达差异的分析[J].肿瘤防治研究,2012,39(8):947-949,3.
