中南民族大学学报：自然科学版2012，Vol.31Issue(3)：33-37,5.
山芝烯二醇对体外培养成骨细胞成骨活性的影响
Effects of Serratenediol on the Osteogenic Activity of Cultured Osteoblasts in vitro
摘要
Abstract
To study the effect of serratenediol(an extract from Lycopodium obscurum L.) on the osteogenic activity of cultured osteoblasts,MTT assay、 alkaline phosphate(ALP) assay kit and real-time PCR were used to determine the proliferation rate,ALP activity and osteogenic genes(c-jun、c-fos、Osterix、ALP、Col-Ⅰ、OC) expression of osteoblasts.The results elucidated that treatment of serratenediol for 3 days could promote the proliferation rate,the expression of c-jun and c-fos genes and first inhibit then enhance Osterix gene of osteoblasts.Serratencdiol treatment for 9 days could enhance the expression of ALP and Col-Ⅰ genes.There was no obvious effect of serratenediol on the expression of OC.So serratenediol could promote the proliferation rate,ALP activity and some osteogenic gene expression of osteoblasts.It is one of the effective component from Lycopodium obscurum L.for bone healing.关键词
山芝烯二醇/成骨细胞/成骨活性Key words
serratenediol/osteoblast/osteogenic activity分类
生物科学引用本文复制引用
王朝元,唐俊龙,魏甜甜,宋超,杨光忠..山芝烯二醇对体外培养成骨细胞成骨活性的影响[J].中南民族大学学报：自然科学版,2012,31(3):33-37,5.基金项目
湖北省自然科学基金资助项目 （）
