中山大学学报（自然科学版）2012，Vol.51Issue(5)：93-99,7.
一株产纤维素酶细菌的分离鉴定及其酶学特性研究
Isolation and Identification of a Strain of Cellulase-Producing Bacterium and Its Enzymatic Characteristics
摘要
Abstract
Using CMC plat screening and Congo red dying methods, a strain of cellulase-producing bacterium named CXB001 was isolated from the soil around rotten root of Hibiscus mutabili on the campus of Hunan University of Science and Technology. Physical and chemical test and molecular phylogenetic a-nalysis based on 16S rDNA sequences showed that the strain was Bacillus velezensis. Biological charateris-tics revealed that the optimal growth temperature is 37℃ , and the growth temperature ranges from 10℃ to 50℃ , while the growth pH ranges from 5.0 to 11.0. The culture conditions at pH 6.0-9.0, 34 ~ 40 ℃ and 1.5% ~3. 5% NaCl are the most suitable for enzyme production. The optimal temperature for CXB001 to produce cellulase is 50℃ , while the optimal reaction pH is 5. 0, and the strain has nice enzyme stability in 20℃, pH 5. 0 ~7.0. Further experiments revealed that Co2+ could stimulate the cellulase, while Mg2+ and Fe2+ inhibit the enzyme reaction.关键词
纤维素酶/Bacillus velezensis/16S rDNA/酶学特性Key words
cellulase / Bacillus velezensis/ 16S rDNA / enzymatic characteristics分类
生物科学引用本文复制引用
邓先余,邹谋勇,黄志坚,易俗..一株产纤维素酶细菌的分离鉴定及其酶学特性研究[J].中山大学学报（自然科学版）,2012,51(5):93-99,7.基金项目
国家“863”计划资助项目(2005AA219040) （2005AA219040）
华南农业大学农业部生态农业重点开放实验室开放基金资助项目(K11) （K11）
湖南省大学生创新性实验资助项目 （）
