组织工程与重建外科杂志2012，Vol.8Issue(4)：185-188,4.DOI:10.3969/j.issn.1673-0364.2012.04.002
葡萄糖和谷氨酰胺浓度对人脐带间充质干细胞体外培养的影响
Effects of Various Concentrations of Glucose and Glutamine on the Cultivation of Human Umbilical Cord-derived Mesenchymal Stem Cells in Vitro
摘要
Abstract
Objective To explore the effects of various concentrations of glucose and glutamine on the cultivation of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in vitro. Methods The hUC-MSCs were separated and cultured. The morphology and surface antigens were investigated by optical microscopy and flow cytometry. Then hUC-MSCs were cultured with various concentrations of glucose and glutamine in vitro. Cells on microcarriers were counted by crystal violet staining. The nutrients and their by-products in the medium supernatant were measured. Results The markers of CD29, CD44, CD105, and CD90 were strongly expressed in hUC-MSCs, but the markers of CD14 and CD45 were not. With the increase of glucose concentration, the expansion of cells increased, glucose consumption, lactate formation and glutamine consumption had no change, and the ammonium formation improved. The increase of glutamine concentration had a limited effects to improving cell proliferation, but it shorted the culture period as improving growth rate. The increase of glutamine concentration had no effect on the consumption of glucose and glutamine, but improved the formation of lactate and ammonium. Conclusion Glucose and glutamine both enhanced cell proliferation. Lack of glucose results in the larger consumption of amino acids to provide nitrogen, and large supply of glutamine leads to the larger formation of lactate from glutamine.关键词
脐带间充质干细胞/葡萄糖/谷氨酰胺/生长/代谢Key words
Umbilical cord-derived mesenchymal stem cells/Glucose/Glutamine/Growth/Metabolism分类
生物科学引用本文复制引用
张淑香,陈彦田,齐念民..葡萄糖和谷氨酰胺浓度对人脐带间充质干细胞体外培养的影响[J].组织工程与重建外科杂志,2012,8(4):185-188,4.基金项目
中国博士后基金面上项目(2011M500780). （2011M500780）
