癌变·畸变·突变2012，Vol.24Issue(5)：335-339,5.DOI:10.3969/j.issn.1004-616x.2012.05.003
甲氧化三丁基锡的急性毒性和遗传毒性研究
Acute toxicity and genetic toxicity of tributyltin methoxide
摘要
Abstract
OBJECTIVE: To investigate the acute toxicity and genetic toxicity of tributyltin methoxide (TBTMO). METHODS: A total of 110 Kunming mice were randomly divided into 11 groups, then treated orally with various doses of TBTMO (420, 286, 194, 180, 132, 90, 73, 61, 41.5 and 28 mg/kg) to perform acute toxicity test. A total of 50 Kunming mice were randomly divided into 5 groups (TBTMO 80, 40, 20 mg/kg, positive control and negative control). Positive control was treated for five consecutive days. Other four groups were treated for two days. These animals were killed 35 d later, and their bilateral epididymis were removed for sperm shape abnormality test. Another 50 Kunming mice were randomly divided into 5 groups and treated as above mentioned. The animals were killed 30 h after gavage with TBTMO, and their chest bone marrow fluid were obtained for bone marrow micronucleus test. Another 50 GFP transgenic mice were randomly divided into 5 groups and treated as above. The animals were killed and their spleen lymphocyte was used for hprt gene mutation test. Various doses of TBTMO (69.4, 48.6, 34.0, 23.8, 16.6 and 0 μg/ml) were used to treat Chinese hamster lung (CHL) cell for chromosome aberration test. Different doses of TBTMO (5×l05, 5×l04, 5×l03, 5×l02, 50, 5, 0.5, 0.05, 5×l0-3 5 × 10 4 |JL g/dish) were used to deal with TA97, TA98, TA100, TA102 strains for Ames test. RESULTS: The LD50 of TBTMO in mice was 98 mg/kg. TBTMO could induce sperm abnormality in a dose-dependent manner (P < 0.05). The other four tests showed negative results. CONCLUSION: Under our experimental conditions, TBTMO was classified as moderately-toxic substance. It may produce genetic toxicity.关键词
甲氧化三丁基锡/急性毒性/遗传毒性Key words
tributyltin methoxide/ acute toxicity/ genetic toxicity分类
生物科学引用本文复制引用
刘文斌,杨录军,李永红,刘晋祎,曹佳,敖琳..甲氧化三丁基锡的急性毒性和遗传毒性研究[J].癌变·畸变·突变,2012,24(5):335-339,5.基金项目
国家重点基础研究发展计划(2011CB503705) （2011CB503705）
