癌变·畸变·突变2012，Vol.24Issue(5)：349-351,3.DOI:10.3969/j.issn.1004-616x.2012.05.006
微囊藻毒素-LR对PC12细胞氧化性损伤作用的实验研究
Preliminary study on toxic effects of microcystins-LR in PC12 cells
摘要
Abstract
OBJECTIVE: To study the toxic oxidative damage in PC12 cells caused by microcystins-LR(MC-LR). METHODS: MTT assays and lactate dehydrogenase (LDH) in culture medium of PC 12 cells were performed to assess the toxicity of MC-LR. The contents of MDA, SOD, GSH-Px and CAT were measured with kits. RESULTS: MTT assay showed that MC-LR had an inhibitory effect on PC 12 cells, and the IC50 of MC-LR were 89.06, 47.24, 33.13 μg/L at l2h, 24 h and 48 h, respectively. Twenty-four hours after treatment by MC-LR, the production of LDH in culture medium was increased. Compared with control group, the contents of MDA and SOD were apparent when MC-LR was more than 10 μg/L (P<0.05). The catalase activity change was similar to SOD. GSH-Px content showed no significant difference compared with the control group. CONCLUSION: MC-LR could inhibit proliferation and induce membrane damage and oxidative damage in PC 12 cells.关键词
微囊藻毒素/神经毒性/PC12细胞/乳酸脱氢酶/氧化损伤Key words
microcystin-LR/ neurotoxicity/ PC12 cells/ lactate dehydrogenase/ oxidative damage分类
医药卫生引用本文复制引用
周珏,刘冉,李晓波,杨菲飞,李云晖..微囊藻毒素-LR对PC12细胞氧化性损伤作用的实验研究[J].癌变·畸变·突变,2012,24(5):349-351,3.基金项目
国家自然科学基金(81072258),江苏省自然科学基金(BK2009281,BK20100402) （81072258）
