安徽农业科学2012，Vol.40Issue(24)：11949-11950,11956,3.
基于Red重组系统进行基因替换的方法建立
A Method for Establishment of Gene Replacement Based on Red Recombinant System
摘要
Abstract
The rapid and highly-efficient gene replacing is prerequisite for studying E. coli gene engineering strains. The Red recombinant system was combined with genetic engineering techniques to substitute chloromycetin-resistant gene from genetic engineering strain and simultaneously maintain galactose-screening marker. We have successfully confirmed that chloromycetin-resistant gene could be replaced by employing Red recombinant system and simultaneously retained other characteristic of strain, thus providing important insights inlo genetic engineering strain with new genetic characteristics and gene function research.关键词
Red重组系统/基因替换/筛选标记Key words
Red recombination system/ Gene replacement/ Screening marker分类
农业科技引用本文复制引用
黄晓萍,郭建军,钟青,罗成..基于Red重组系统进行基因替换的方法建立[J].安徽农业科学,2012,40(24):11949-11950,11956,3.基金项目
江西省科技支撑计划(20111BDH80035,20114BAB204016) （20111BDH80035,20114BAB204016）
江西省科学院项目(2010-YGJ-01,2010-YGJ-02). （2010-YGJ-01,2010-YGJ-02）
