安徽农业科学2012，Vol.40Issue(24)：12075-12078,4.
三氧化二砷对大鼠成骨细胞增殖及BMP-2的影响
Effects of Arsenic Trioxide on the Proliferation and BMP-2 in Osteoblasts Cultured in vitro
摘要
Abstract
[ Objective] To study the effects of different doses of arsenic trioxide on the proliferation and bone morphogenetic protein -2 (BMP-2) gene expression of cultured rat osteoblasts cells. [Method] After the osteoblasts were treated by different concentrations of As2O3(10, 1 and 0. lμmol/L) for 48 h and 72 h, the morphology of osteoblasts was observed under inverted microscophy, the proliferation of osteoblasts was tested by MTT assay, and the BMP-2 gene expression of osteoblasts was determined with RT-PCR. [ Result] In 10 μmol/L arsenic trioxide treatment group, some osteoblasts become rounder and smaller, the nuclear was condensed and fragmented, and the cell membrane folded and depressed; in 1 μmol/L arsenic trioxide treatment group, there were large number of osteoblasts with obvious split phase; inO. 1 μmol/L arsenic trioxide treatment group, the osteoblasts were closely connected. The OD value of 10 μmol/L group was significantly lower than the control group, OD value in 72 h was lower than 48 h, which had statistical difference(P <0.01) ;OD value of 1 μmol/L group was significantly higher than the control group. OD value in 72 h was higher than OD value in 48 h .which had statistical difference( P <0.01). The OD value of 0. 1 μmol/L group in 72 h had no statistical difference with 48 h( P > 0.05 ) and was the same as control group. The BMP-2 gene expression of 10 μmol/L group in 72 g was significantly lower than in 48 g and BMP-2 gene expression of 10 μmol/L group was significantly lower than the control group, which had statistical difference(P<0.01) ; BMP-2 gene expression of 1 μmol/L group had no significant difference between in 48 h and in 72 h and the same as control group(P >0.05). BMP-2 gene expression of 0.1 μmol/L group in 72 h was significantly higher than in 48 h and BMP-2 gene expression of 10 μmol/L group was significantly higher than the control group, which had statistical differ-ence(P<0.01). [Conclusion] As2O3 has a positive-negative regulatory role for cell proliferation and BMP-2 mRNA expression to rat osteo-blast in vitro, and presents a dose- time dependence.关键词
As2O3/成骨细胞/增殖/BMP-2Key words
Arsenic trioxide/ Osteoblasts/ Proliferation/ BMP-2分类
农业科技引用本文复制引用
陆祥,范桂金,陈伟,何志全..三氧化二砷对大鼠成骨细胞增殖及BMP-2的影响[J].安徽农业科学,2012,40(24):12075-12078,4.基金项目
遵义医学院硕士启动基金项目(F-351). （F-351）
