安徽农业科学2012，Vol.40Issue(24)：12082-12084,3.
猪源ETEC的黏附素基因克隆与原核表达
Gene Cloning and Prokaryotic Expression of Adhesin in Enterotoxigenic Escherichia coil from Swine
徐刚 1高继业 2郎静宇 2唐妤 2李阁 3李继祥2
作者信息
- 1. 成都农业科技职业学院,四川温江611130
- 2. 西南大学荣昌校区,重庆402460
- 3. 重庆永健生物技术有限公司,重庆402460
- 折叠
摘要
Abstract
[ Objective] The research aimed to overcome the disadvantages of the traditional preparation method of adhesion. [ Method] The gene fragments offaeG fasC and fasG were amplified by PCR from the plasmid of adhesin (F4, F5 and F6) of enterotoxigenic E. coli(ETEC) and sequenced to construct the recombinant expression vectors with pET 32a ( + ). The recombinant expression vectors were transferred into E. coli BL 21 (DE3) to analyze their immunogenicity. [Result] The recombinant expression vectors were highly expressed by IPTG induction. The results of hemagglutination inhibition assay showed mice antiserum could inhibit the standard agglutinate erythrocyte of highly virulent strain of ETEC and the titer was above 1:128, which indicated that the cloned and expressed adhesion protein of swine ETEC had a good immunogenicity. [ Conclusion] The research could lay the foundation for further development of antibody preparation.关键词
产肠毒素性大肠杆菌/黏附素/克隆/原核表达/免疫原性Key words
Enterotoxigenic Escherichia coli/ Adhesin/Cloning/Prokaryotic expression/Immunogenicity
分类
农业科技引用本文复制引用
徐刚,高继业,郎静宇,唐妤,李阁,李继祥..猪源ETEC的黏附素基因克隆与原核表达[J].安徽农业科学,2012,40(24):12082-12084,3.
