安徽农业科学2012，Vol.40Issue(28)：13787-13789,3.
S-8大孔吸附树脂分离纯化洒金桃叶珊瑚中桃叶珊瑚苷的工艺研究
Study on Separation and Purification of Aucubin from Aucuba japonica Variegata with S-8 Macroporous Resin
摘要
Abstract
[Objective] To study the separation and purification process of aucubin with macroporous resin, so as to lay foundation for preparing aucubin from Aucuba japonica Variegata. [ Method] Taking aueubin as index, the concentration of aucubin was measured by HPLC to optimize the best separation and purification conditions of aucubin with macroporous resin. [ Result] The optimum conditions of S-8 macroporous resin were as follows; sample concentration of 2.76 mg/ml, sample flow velocity of 2 BV/h, sample capacity of 9.20 mg/g, elutant of 15% ethanol aqueous, elution flow rate of 2 BV/h, and total quantity of eluant of 3 BV. [ Conclusion] It has good effect to separate aucubin by S-8 macroporous resin, the content and recovery rate of aucubin were 48. 04% and 82. 30% .关键词
大孔吸附树脂/桃叶珊瑚苷(aucubin)/纯化Key words
Macroporous resin/ Aucubin/ Purification分类
农业科技引用本文复制引用
龙桢桢,乔媛媛,李静,郑敬,魏屹,成晓梅..S-8大孔吸附树脂分离纯化洒金桃叶珊瑚中桃叶珊瑚苷的工艺研究[J].安徽农业科学,2012,40(28):13787-13789,3.基金项目
四川省科技支撑项目(2008SZ0009) （2008SZ0009）
中央高校基本科研专项(NO:SWJTU11ZT25). （NO:SWJTU11ZT25）
