安徽农业科学2012，Vol.40Issue(28)：13955-13956,13959,3.
溶藻细菌H5的分离、鉴定及溶藻特性研究
Isolation,Identifcation and Characterization of Algae-lysing Strain H5 from Xiangxi Bay of Three Gorges Reservoir
摘要
Abstract
[Objective] The study was conducted to isolate and identify alage-lysing bacterium and explore the characterization of alage-lysing bacterium so as to provide reference for the control of algae bloom. [ Method] A high-efficient algae-lysing strain H5 was isolated during spring blooms in Xiangxi Bay at Three Gorges Reservoir. According to the similarity analysis of its 16S rRNA gene sequence and by Biolog microsta-tion system, the strain was identified. By direct counting, the lytic efficiency of H5 to Stephanodiscus hantzschii, Peridiniopsis niei, Komma caudata and performing mode of H5 to S. hantzschii were studied. [ Result] 16S rDNA sequence analysis and Biolog analysis showed that H5 strain belonged to Lysinibacillus fusiformis. The highest and lowest algicidal rate of the strain was 71.3% and 57.4% respectively in the cultures of 5. hantzschii, P. niel, and K. caudata. The filtrate of cell spent media, and the heated above filtrate displayed the same algae-lytic ability, while the cell-free supernatant of the cells of bacteria showed no algae-lytic ability, indicating that some extracellular and thermo-stable substances were produced by this strain. [Conclusion] The strain H5 produced better lytic efficiency to S. hantzschii and performed algae-lytic ability by producing extracellular substances.关键词
溶藻细菌/芽孢杆菌/水华/汉斯冠盘藻/香溪河Key words
Algae-lysing bacterium/ Lysinibacillus fusiformis/ Bloom/ Stephanodiscus hantzschii/ Xiangxi River分类
农业科技引用本文复制引用
刘国勇,胡亚平,石小丹,聂小倩,黄应平..溶藻细菌H5的分离、鉴定及溶藻特性研究[J].安徽农业科学,2012,40(28):13955-13956,13959,3.基金项目
国家自然科学基金项目(50979049,51179096) （50979049,51179096）
三峡大学博士启动基金项目(KJ2008B016). （KJ2008B016）
