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地鳖虫多糖提取工艺的优化研究

罗志文 刘娟 张聪颖 赵永勋

安徽农业科学2012,Vol.40Issue(28):13978-13979,13982,3.
安徽农业科学2012,Vol.40Issue(28):13978-13979,13982,3.

地鳖虫多糖提取工艺的优化研究

Optimization of the Extraction Technology of Eupolyphaga sinensis Polysaccharide

罗志文 1刘娟 2张聪颖 2赵永勋1

作者信息

  • 1. 佳木斯大学生命科学学院,黑龙江佳木斯154007
  • 2. 佳木斯大学药学院,黑龙江佳木斯154007
  • 折叠

摘要

Abstract

[ Objective ] To extract Eupolyphaga sinensis polysaccharide through experiment for the research and development of polysaccharides in traditional Chinese medicine. [ Method] The Eupqlyphaga sinensis polysaccharides were extracted by hot water, and the extraction conditions were determined by single factor test, an orthogonal experiment was carried out with the four factors of extraction time, solid-liquid ratio, extraction temperature and extraction times, the polysaccharides content was determined using phenol-sulfuric acid. [ Result] Extraction temperature had the largest impact on the extraction of Eupolyphaga sinensis polysaccharides, then followed by extraction time, extraction time and solid-liquid ratio. The optimal extraction conditions were 1.5 h of extraction time, l:10(g/ml) of solid-liquid ratio, 80 X of extraction temperature , and three times of extraction. [ Conclusion] The main factors influencing the extraction of insect water soluble polysaccharides included extraction temperature, extraction time, extraction times and solid-liquid ratio. With the prolonging extraction time, the cells disrupted completely , more polysaccharide was dissolved out, and the polysaccharides content improved.

关键词

地鳖虫多糖/热水浸提法/提取工艺/提取条件优化

Key words

Eupolyphaga sinensis polysaccharide/ Hot water extraction/ Extraction process/ Optimization of extraction conditions

分类

农业科技

引用本文复制引用

罗志文,刘娟,张聪颖,赵永勋..地鳖虫多糖提取工艺的优化研究[J].安徽农业科学,2012,40(28):13978-13979,13982,3.

基金项目

黑龙江省教育厅科学技术项目(11531384). (11531384)

安徽农业科学

0517-6611

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