安徽农业科学2012，Vol.40Issue(29)：14185-14187,14270,4.
小叶满江红(Azolla microphylla)内生细菌多样性的T-RFLP分析
T-RFLP Analysis on Diversity of Endophytic Bacteria in Azolla microphylla
摘要
Abstract
[ Objective ] To explore the diversity of endophytic bacteria in Azolla microphylla by using T-RFLP. [ Method ] PCR products of DNA of endophytic bacteria in Azolla microphylla which were cultured by our laboratory in sterile conditions were digested with restriction endoenzyme Mspl, Hhal, Haelll, Hind, respectively. The terminal fragment was analyzed with fluorescent through Phylogenetic Assignment Tool (PAT). [ Result ] Bacteria belong to 10 Phylums 42 Classes were gained after analysis. [ Conclusion ] The genetic diversity of endophytic bacteria in Azolla microphylla could be figured out by using T-RFLP and bioinformatic software.关键词
满江红/内生细菌/T-RFLPKey words
Azolla microphyllla/ Endophytic bacteria/ T-RFLP分类
农业科技引用本文复制引用
郑斯平,陈彬,王瑾,陆培基..小叶满江红(Azolla microphylla)内生细菌多样性的T-RFLP分析[J].安徽农业科学,2012,40(29):14185-14187,14270,4.基金项目
福建省科技厅公益项目(2010R1021-8). （2010R1021-8）
