安徽农业科学2012，Vol.40Issue(29)：14191-14194,4.
甘蔗试管苗瓶外生根技术的研究
Studies on ex vitro Rooting of Sugarcane Microshoots
摘要
Abstract
[Objective ] To optimize the ex vitro rooting conditions of sugarcane microshoots, improve their survival rate and reduce the production costs. [Method] The effect of external environmental factors on the ex vitro rooting and growth of sugarcane microshoots was studied, the survival rate and plant height of in. vitro and ex vitro rooting were compared. [ Result ] The high fertile substrate ( commercial) was not beneficial for higher survival rate but promoted the growth of plants, while the lower fertile substrate ( sand) was not beneficial for growth of shoot, but resulted in higher survival rate. The temperature, humidity and light intensity of ex vitro microenvironment should be changed gradually to natural ones during acclimatization through covering of plastic film and shading net and proper water management after transplanting so that sugarcane micro-shoot could be well adjusted to natural conditions at last. In addition, the in vitro rooting shoots showed higher survival rate and better growth than the ex vitro shoots. [Conclusion] The current study will help to develop the techniques for attaining higher survival rate of micro shoots and ultimately reduce the cost of large scale production of disease-free tissue cultured sugarcane plantlets.关键词
甘蔗/无根试管苗/瓶外生根/存活率Key words
Sugarcane/ Microshoots/ ex vitro rooting/ Survival rate分类
农业科技引用本文复制引用
何为中,刘红坚,李松,汪淼,翁梦苓,王天算,韦明珠,苏树权,余坤兴..甘蔗试管苗瓶外生根技术的研究[J].安徽农业科学,2012,40(29):14191-14194,4.基金项目
广西自然科学基金项目(桂科回0991012) （桂科回0991012）
公益性科研院所基本科研业务项目(G2009013) （G2009013）
广西科学研究与技术开发项目(桂科合1140011-3) （桂科合1140011-3）
公益性科研院所基本科研业务项目(G2010011). （G2010011）
