乳糖诱导成纤维细胞生长因子-21在大肠杆菌中的表达OA
Expression of Fibroblast Growth Factor 21 in E. coli Induced with Lactose
[目的]研究用乳糖作为诱导剂诱导成纤维细胞生长因子-21(fibroblast growth factor 21,FGF21)基因重组蛋白的表达,以探讨其工程化生产的可能性.[方法]对影响重组表达产物的乳糖诱导浓度、诱导时机、诱导时间及诱导温度等进行比较,并以IPTG诱导作为对照,确定最佳的乳糖诱导条件.[结果]对于重组目的蛋白,乳糖可以起到很好的诱导效果,诱导产物的表达量与IPTG诱导产物相当.其最佳表达条件为:在A600为0.8～1.0时,加…查看全部>>
[ Objective] To study the expression of fibroblast growth factor-21 induced with lactose, and explore the possibility of engineering production. [Method] The effects of the concentration, induction time and induction temperature of lactose on the expression of the recombi-nant product were compared and IPTG induction was used as a control to determine a more optimal conditions for lactose induction. [ Result] For the production of recombinant FGF21, lactose …查看全部>>
王会岩;尹彬彬;明盛金;李俊;宋莹;王蕾;尹维笳;蔡建辉
吉林医药学院,吉林吉林132013吉林医药学院,吉林吉林132013吉林医药学院,吉林吉林132013吉林医药学院,吉林吉林132013吉林医药学院,吉林吉林132013吉林医药学院,吉林吉林132013吉林医药学院,吉林吉林132013吉林医药学院,吉林吉林132013
农业科技
成纤维细胞生长因子-21乳糖诱导大肠杆菌
Fibroblast growth factor-21 Lactose induction Escherichia coli
《安徽农业科学》 2012 (30)
14636-14638,3
吉林省科技发展计划项目资助课题(20090949,20116044)吉林医药学院大学生科研基金(课题编号:吉医学科字[2010]第09号).
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