安徽农业科学Issue(31)：15123-15126,4.
美洲黑杨及其杂交种的遗传多样性AFLP分析
Genetic Diversity of Populus deltoides Marsh. and Hybrids Clones by AFLP
徐金光 1王玮 1刘霞 2刘莉娟 3王东升 3桑亚林 3邢世岩 3孟宪伟4
作者信息
- 1. 山东省林木种苗站,山东济南250014
- 2. 河北农业大学林学院,河北保定071000
- 3. 山东农业大学林学院,山东泰安271018
- 4. 宁阳县国有高桥林场,山东泰安271416
- 折叠
摘要
Abstract
The aim was to analyze the genetic diversity of Populus deltoids Marsh, and its hybrids clones by AFLP technique. [ Method] The genetic diversity and relationship of 63 Populus L. clones were tested by AFLP molecular marker technique, and then the hierarchical diagram was constructed. [ Result] PCR was done with the 63 Populus L. clones by the selected 8 primers and a total of 1 153 fragments were produced, 1 152 of which were polymorphic with a polymorphism percentage of 99.92% , this result showed that there were abundant diversities in enzyme digestion sites among different Populus L. clones in the DNA level. There were a total of 165 specific bands, including 14 missing bands. The molecular genetic similarity coefficients ranged from 0.077 9 to 0. 794 6, and the average being 0. 548 7 indicated that genetic variation occurred among them. All of Populus L. were clustered into 7 groups with UPGMA, and the same species didn' t distribute in the same group toally. [ Conclusion] It prepared utility reference in molecular level for the evaluation, protection and utilization of Populus deltoids Marsh, germplasm resource.关键词
美洲黑杨/杂交种/遗传多样性/AFLPKey words
Populus deltoids Marsh. / Hybrids clones/ Genetic diversity/ AFLP分类
农业科技引用本文复制引用
徐金光,王玮,刘霞,刘莉娟,王东升,桑亚林,邢世岩,孟宪伟..美洲黑杨及其杂交种的遗传多样性AFLP分析[J].安徽农业科学,2012,(31):15123-15126,4.
