安徽农业科学Issue(31):15142-15143,2.
芦笋花药愈伤组织诱导技术研究
Preliminary Study on Anther Culture Callus Inducement of Asparagus
摘要
Abstract
To study anther culture callus inducement of asparagus in detail. [Method] The diploid anther callus inducement was studied by MS as basic medium with exogenous hormone. [ Result] The length of the diploid bud was 1. 5 - 2. 0 mm which induced result best; The high sugar density of 5% can repress the anther somatic cell division remarkably and improved the inducement rate of pollens callus; The callus inducement rate of the same plant with different species differed greatly; The suitable hormone proportion of diploid anther callus was 1.0 mg/L NAA + 1.0 mg/L BA. [ Conclusion] The study will lay a foundation for improvement and breeding of asparagus.关键词
芦笋/花药培养/愈伤组织/诱导Key words
Asparagus/ Anther culture/ Callus/ Inducement分类
农业科技引用本文复制引用
张元国,李芳,包艳存..芦笋花药愈伤组织诱导技术研究[J].安徽农业科学,2012,(31):15142-15143,2.基金项目
山东省农业良种产业化项目(wkns200202). (wkns200202)