安徽农业科学Issue(31):15274-15276,3.
利用绒山羊原始生殖细胞培养胚胎干细胞的研究
Study on Embryonic Stem Cell from Cultured Primordial Germ Cells of Down Producing Goat
摘要
Abstract
To establish embryonic stem cell system of goats. [Method] Numerous primordial germ cells colonies were derived from gonadal ridge and the surrounding tissues 20 millimeter fetuses of down producing goat. Primordial germ cells and goats embryonic fibro-blasts obtained from a conceptus of equivalent gestational age were co-cultured. [Result] The colonies had some characteristics of embryonic stem cells, such as the morphology of nest-like, they continued to be AKP positive and the ability to be continuously passed. [ Conclusion ] These cells were pluripotent and ES-like cells.关键词
绒山羊/原始生殖细胞/胚胎干细胞Key words
Down producing goat/ Primordial germ cell/ Embryonic stem cells分类
农业科技引用本文复制引用
图雅,卢玲..利用绒山羊原始生殖细胞培养胚胎干细胞的研究[J].安徽农业科学,2012,(31):15274-15276,3.基金项目
包头师范学院校内项目(BSY2010-23). (BSY2010-23)