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利用绒山羊原始生殖细胞培养胚胎干细胞的研究

图雅 卢玲

安徽农业科学Issue(31):15274-15276,3.
安徽农业科学Issue(31):15274-15276,3.

利用绒山羊原始生殖细胞培养胚胎干细胞的研究

Study on Embryonic Stem Cell from Cultured Primordial Germ Cells of Down Producing Goat

图雅 1卢玲1

作者信息

  • 1. 包头师范学院生物科学与技术学院,内蒙古包头014030
  • 折叠

摘要

Abstract

To establish embryonic stem cell system of goats. [Method] Numerous primordial germ cells colonies were derived from gonadal ridge and the surrounding tissues 20 millimeter fetuses of down producing goat. Primordial germ cells and goats embryonic fibro-blasts obtained from a conceptus of equivalent gestational age were co-cultured. [Result] The colonies had some characteristics of embryonic stem cells, such as the morphology of nest-like, they continued to be AKP positive and the ability to be continuously passed. [ Conclusion ] These cells were pluripotent and ES-like cells.

关键词

绒山羊/原始生殖细胞/胚胎干细胞

Key words

Down producing goat/ Primordial germ cell/ Embryonic stem cells

分类

农业科技

引用本文复制引用

图雅,卢玲..利用绒山羊原始生殖细胞培养胚胎干细胞的研究[J].安徽农业科学,2012,(31):15274-15276,3.

基金项目

包头师范学院校内项目(BSY2010-23). (BSY2010-23)

安徽农业科学

0517-6611

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