安徽医科大学学报2012，Vol.47Issue(11)：1300-1303,4.
FasL反义寡核苷酸对舌鳞癌细胞Tca8113增殖和凋亡的影响
Effect of FasL antisense oligonucleotide on proliferation and apoptosis of Tca8113
摘要
Abstract
Objective To investigate the effect of Fas ligand( FasL ) antisense oligonucleotide( ASO ) on proliferation and apoptosis of Tca8113 cells in vitro. Methods Specific phosphorothioate ASO were synthesized and trans-fected into Tca8113 cells with lipofectamine 2000. The growth and the transfection of Tca8113 cells were observed by inverted microscope and the transfection efficiency was tested by flow cytometry( FCM ). The cell viability of Tca8113 cells was assessed by methyl thiazolyl tetrazolium ( MTT ) assay and the apoptosis rate was detected by FCM before- and after-transfection. Results The FasL ASO inhibited cell proliferation in a dose- and time-dependent manner within limits. The total apoptosis rate was apparently increased( P <0. 05 ) in antisense group compared with the control group,the lipofectamine group and the sense group. Conclusion FasL ASO can suppress proliferation and induce significant apoptosis of Tca8113 cells. FasL ASO may become a sort of gene therapy of tongue squamous cell carcinoma.关键词
Fas/Fas 配体/反义寡核苷酸技术/舌鳞癌细胞Key words
Fas/FasL/ antisense oligonucleotide/Tca8113分类
医药卫生引用本文复制引用
方 丽,陈乔尔,任翠平,沈际佳..FasL反义寡核苷酸对舌鳞癌细胞Tca8113增殖和凋亡的影响[J].安徽医科大学学报,2012,47(11):1300-1303,4.基金项目
安徽省自然科学基金(编号:11040606M175) （编号:11040606M175）
