安徽医科大学学报2012，Vol.47Issue(11)：1351-1355,5.
TMPRSS2-ERG、AMACR和p63在前列腺癌中的表达及相关性
Expression of TMPRSS2-GER, AMACR and p63 in prostate cacinoma and their correlation
摘要
Abstract
Objective To investigate the expression of TMPRSS2-ERG, AMACR and p63 in prostate and their cor- relation with clinicopathological factors. Methods Immunohistochemistry was used to detect the expression of AM-ACR and p63 and fluorescence in situ hybridization( FISH ) was used to detect TMPRSS2-ERG gene fusion in 50 cases of prostate carcinoma, 30 cases of benign prostatic hyperplasia, 10 cases of high grade prostatic intraepithelial neoplasia( HGPIN ) and 10 cases of prostate metastatic cancer tissue, then their correlation was analyzed. Results The expression of TMPRSS2-ERG gene fusion and AMACR was higher in prostate carcinoma but lower in benign prostatic hyperplasia tissues( P <0. 01 ). And p63 was lower in prostate carcinoma and prostate metastatic cancer than benign prostatic hyperplasia and high grade prostatic intraepithelial neoplasia( P < 0. 01 ). TMPRSS2-ERG gene fusion was stably highly expressed in prostate carcinoma, and the expression was not related with the degree of prostate-specific antigen, Gleason score and TNM, however, p63 and AMACR were highly related ( P < 0. 05 ). Statistical correlation was found between the expression of TMPRSS2-ERG and AMACR ( P < 0. 05 ). And the coincidence rate was enhanced with the increase of signal in AMACR, and the negative coincidence rate was 71. 4%. Conclusion In the diagnosis of prostate cancer, TMPRSS2-ERG gene fusion is considered to be an independent detection factor;TMPRSS2-ERG and AMACR have a high coincidence rate. TMPRSS2-ERG, AMACR and p63 in prostate carcinoma progression may have a synergistic effect. Joint detection of TMPRSS2-ERG, AMACR and p63 can make a more reliably early diagnosis of prostate cancer in the levels of gene and protein.关键词
前列腺癌/免疫组织化学染色法/荧光原位杂交/TMPRSS2/ERG融合基因/AMACR/p63Key words
prostate carcinoma/ immunohistochemistry/ fluorescence in situ hybridization/ TMPRSS2-ERG gene fusion/ AMACR/ p63分类
医药卫生引用本文复制引用
孙文国,夏利,蒋雷鸣,杨伟娇,莫文发..TMPRSS2-ERG、AMACR和p63在前列腺癌中的表达及相关性[J].安徽医科大学学报,2012,47(11):1351-1355,5.基金项目
广西桂林市科学研究与技术开发计划(编号:20120121-1-2) （编号:20120121-1-2）
