草业学报2012，Vol.21Issue(5)：107-113,7.
3种生态型野生扁蓿豆种质资源ISSR与SSR遗传多样性分析
The genetic diversity of three ecological Medicago ruthenica germplasms revealed by ISSR and SSR
摘要
Abstract
Quantitative and classification was based on germplasm collection and utilization. The genetic diversity of 14 wild Medicago ruthenica was assessed by screening with 18 pairs of SSR and 18 ISSR primers. SSR primers amplified 109 clear polymorphic bands, and the polymorphism rate was 80. 09% with a similarity coefficient ranging from 0. 669 to 0. 885. ISSR primers amplified 125 polymorphic bands and the polymorphism ratio was 87. 08% with a similarity coefficient ranging from 0. 448 to 0. 811. UPGMA (Unweighted Pair Group Method with Arithmetic mean) cluster analysis based on two markers showed that part of the M. ruthenica material grouped into one group. Mantel tests showed that the genetic similarity of two markers had a significant correlation (r=0. 019 6, t = 0. 121 2). Compared with yellow M. ruthenica genetic diversity levels, those of M. ruthenica were higher. The genetic difference was highest in M. ruthenica var. oblongifolia.关键词
扁蓿豆/ISSR/SSR/遗传多样性Key words
Medicago ruthenica /ISSR/SSR/genetic diversity分类
农业科技引用本文复制引用
李鸿雁,李志勇,师文贵,蔡丽艳,张静萍..3种生态型野生扁蓿豆种质资源ISSR与SSR遗传多样性分析[J].草业学报,2012,21(5):107-113,7.基金项目
农业部农作物种质资源保护与利用项目"多年生牧草更新复壮与利用"(NB2011-2130135-33)和农业部牧草种质资源保护项目"牧草温带备份库的维护与备份繁殖入库"(2010-43)资助. （NB2011-2130135-33）
