重庆理工大学学报：自然科学2012，Vol.26Issue(11)：23-26,44,5.
乳酸脱氢酶A（ldha）基因的克隆与原核表达
Cloning and Prokaryotic Expression of Idha Gene
摘要
Abstract
The lactic dehydrogenase is one kind of enzymes in glycolysis.It can catalyze the pyruvic acid into lactic acid under the anaerobic condition.When animal body lacks the glucose,it may also oxidize the lactic acid to pyruvic acid and transform it into glucose by gluconeogenesis in vivo.In this study,ldha gene was obtained from Hela cell by using the reverse transcription polymerase chain reaction(RT-PCR) method.And the protein expression system of Escherichia coli BL21(DE3) for ldha gene was constructed with vector pET28a(+).SDS-PAGE analysis showed that the transformant with recombinant expression plasmid pET28a(+)-ldha produced a protein with corresponding molecular weight of 36 kDa when induced by 1.0 mmol·L-1 IPTG.关键词
Hela细胞/ldha基因/克隆/原核表达Key words
Hela cell/ldha gene/cloning/prokaryotic expression分类
生物科学引用本文复制引用
袁军,许香雅,沈榕强..乳酸脱氢酶A（ldha）基因的克隆与原核表达[J].重庆理工大学学报：自然科学,2012,26(11):23-26,44,5.基金项目
福建省自然科学基金资助项目 （）
福建省教育厅科技计划项目 （）
