重庆医学2012，Vol.41Issue(28)：2911-2913,3.DOI:10.3969/j.issn.1671-8348.2012.28.003
肢体缺血预处理和BQ123药物后处理对大鼠肢体缺血再灌注后肝损伤的影响
Both limb ischemia preconditioning and BQ123 postconditioning have protective effect on hepatic injury following hind limbs ischemia reperfusion in rats
摘要
Abstract
Objective To evaluate the role of limb ischemia preconditioning and BQ123 postconditioning on hepatic injury following hind limbs ischemia reperfusion in rats. Methods Wistar rats were divided into 4 groups(n = 8) :Control group,limbs ische-mia-reperfusion group(LIR) ,limb ischemia preconditioning group(IPC) and BQ123 postconditioning group(BQ123). The changes of Endothelin-l(ET-l) ,malondialdehyde(MDA) ,alanine transaminase(ALT) and aspartate transaminase(AST) in plasma were observed. The contents of ET-1 , MDA,Myeloperoxide(MPO) and the ratio of DNA chain in liver were measured. Results The levels of ET-1 ,MDA, ALT, AST in plasma and the contents of ET-1 ,MDA,MPO in liver increased following LIR.the ratio of DNA chain in liver decreased(P<0. 05) ; Comparing to LIR,the levels of ET-1, MDA, ALT, AST in plasma and the contents of ET-l,MDA, MPO in liver decreased,the ratio of DNA chain in liver increased(P<0.05)both in IPC group and in BQ123 group;there were no significant differences in above indexes between IPC group and BQ123 group(P>0. 05). Conclusion Both limb ischemia preconditioning and BQ123 postconditioning can attenuate liver injury following IR in the hind limbs of rats,the protective effect is perhaps related to restrain the action of ET-1, improving the aggregation of neutrophil and alleviating the damage of the lipid peroxidation.关键词
缺血预处理/BQ123/再灌注损伤/肝损伤/大鼠Key words
ischemia preconditioning/BQ123 /reperfusion injury/liver injury/rats引用本文复制引用
赵林静,王永玲,杨保胜,张金盈..肢体缺血预处理和BQ123药物后处理对大鼠肢体缺血再灌注后肝损伤的影响[J].重庆医学,2012,41(28):2911-2913,3.基金项目
河南省科技厅基金资助项目(411045800). （411045800）
