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乳鼠耳蜗单离外毛细胞胞膜Prestin分布的实验研究

张博 尹时华

重庆医学2012,Vol.41Issue(30):3178-3179,2.
重庆医学2012,Vol.41Issue(30):3178-3179,2.DOI:10.3969/j.issn.1671-8348.2012.30.021

乳鼠耳蜗单离外毛细胞胞膜Prestin分布的实验研究

Experimental study the prestin distribution at the membrane of single outer hair cells of cochlea in newborn rat*

张博 1尹时华1

作者信息

  • 1. 广西医科大学第一附属医院耳鼻咽喉头颈外科,南宁,530021
  • 折叠

摘要

Abstract

Objective To investigate the Prestin distribution at the membrane of single outer hair cells of cochlea in newborn rat. Methods In this experiment,single cell of outer hair cells was geted from 7-day-old wistar rat,the distribution of prestin was examined by im-munofluorescent staining and fluorescence microscopy. Results The staining was expressed on the lateral membrane; the base membrane was also expressed,but weaker;on the bottom side of the membrane and the lateral membrane-binding site,The staining was not expressed. Conclusion Prestin is mainly distributed on the lateral membrane,and basal membrane is also a small amount of expression.

关键词

乳鼠/耳蜗/外毛细胞/Prestin

Key words

newborn rat/cochlea/outer hair cell/ prestin

引用本文复制引用

张博,尹时华..乳鼠耳蜗单离外毛细胞胞膜Prestin分布的实验研究[J].重庆医学,2012,41(30):3178-3179,2.

基金项目

国家自然科学基金资助项目(30860312) (30860312)

国家博士后基金资助项目(20080440819) (20080440819)

广西自然科学基金资助项目(桂科自0728134). (桂科自0728134)

重庆医学

OA北大核心CSCDCSTPCD

1671-8348

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