重庆医学2012，Vol.41Issue(30)：3186-3187,3191,封3,4.DOI:10.3969/j.issn.1671-8348.2012.30.024
水飞蓟素对野百合碱诱发肺动脉高压大鼠肺血管重构的保护作用
Protective effect of Silymarin on rats with monocrotaline induced pulmonary hypertension
杨勇 1吕进泉 1赵文生1
作者信息
- 1. 江苏大学附属医院儿科,江苏镇江,212001
- 折叠
摘要
Abstract
Objective To investigate the protective effects of silymarin on rats with monocrotaline induced pulmonary hypertension and explore its therapeutic mechanism. Methods 24 male SD rats were randomly divided into 3 groups,the control group(n = 8) , the pulmonary hypertension model group(n = 8) and the silmymarin treatment group(n = 8). Right ventricular systolic pressure (RVSP) were detected by right heart catheter after twenty-eight days. Right ventricular hypertrophy index(RVHI) was calculated as the right ventricle to the left ventricular plus septum weight. With HK staining,immunohistochemical analysis of crsmooth muscle actin(crSMA) to observe pulmonary artery structural changes. The pulmonary artery smooth muscle cells apoptosis were detected by TUNEL. The expression of Fas mRNA in the pulmonary tissue was detected by RT-PCR. Results Compared with the model group,silymarin treatment group decreased RVSP,RVHI and α-SMA,increased apoptosis of pulmonary artery smooth muscle cells and Fas mRNA. Conclusion Silymarin could protect rats with monocrotaline induced pulmonary hypertension.
关键词
高血压,肺性/水飞蓟素/野百合碱Key words
hypertension, pulmonary/ silymarin/ monocrotaline引用本文复制引用
杨勇,吕进泉,赵文生..水飞蓟素对野百合碱诱发肺动脉高压大鼠肺血管重构的保护作用[J].重庆医学,2012,41(30):3186-3187,3191,封3,4.
