重庆医学2012，Vol.41Issue(31)：3241-3242,3245,封2,4.DOI:10.3969/j.issn.1671-8348.2012.31.001
新生大鼠海马神经元原代培养及膜片钳全细胞记录
The primary culture for hippocampal neurons of neonatal rats and patch clamp whole-cell recording
摘要
Abstract
Objective To discuss a culture method of hippocampal neurons, which is suitable for patch clamp whole-cell recording. Methods Neonatal Wistar rats(<l d) were decollated and bilateral hippocampus were separated rapidly. Neural basal media supplemented with B-27 and L-glutamine were used for hippocampal neurons primary culture. On day 10,neurons were identified by immunofluorescence of neuronal nuclei(NeuN). Klectrophysiological properties of neurons were recorded by patch clamp whole-cell recording. Results The cultured neurons were in good condition. By identifying, the purity of cultured neurons was almost high to 100%. Action potential and spontaneous excitatory postsynaptic current were recorded by whole-cell patch clamp. Conclusion This is a simple and efficient method, and the cultured neurons are in good condition. Moreover, neurons are suitable for patch clamp whole-cell recording.关键词
大鼠,Wistar/海马/神经元/细胞,培养的/膜片钳术Key words
rats, Wistar/ hippocampus/ neurons/ cells, cultered/ patch-clamp techniques引用本文复制引用
徐祖才,徐平,张骏,雷显泽,徐忠祥,王学峰..新生大鼠海马神经元原代培养及膜片钳全细胞记录[J].重庆医学,2012,41(31):3241-3242,3245,封2,4.基金项目
国家自然科学基金面上项目(81071039) （81071039）
两江学者专项基金 （）
遵义医学院附属医院硕士科研启动基金资助项目(209.001.097.14). （209.001.097.14）
