重庆医学2012，Vol.41Issue(31)：3283-3285,封3,4.DOI:10.3969/j.issn.1671-8348.2012.31.017
硫链丝菌肽对人鼻咽癌HNE-1细胞生长及Foxm1表达的影响
Effect of thiostrepton on proliferation and Foxm1 expression in HNE-1 cell lines
林力 1邓碧 2寿铸 1梁佳 1陈鸿雁1
作者信息
- 1. 重庆医科大学附属第一医院耳鼻咽喉科,400016
- 2. 重庆市渝北区人民医院麻醉科,401120
- 折叠
摘要
Abstract
Objective To explore the effect of thiostrepton(TST) on proliferation and Forkhead box protein Ml (Foxml) expression in HNK-1 cell lines. Methods MTT reduction assay was used to investigate the proliferation inhibition of different concentrations of TST(1 ,2 ,4 ,8,12,16 μmol/L) on HNE-1 cell lines after 48 h. The change of HNE-l's cell cycle after using different concentrations of TST(1,2 μmol/L) were analyzed by flow cytometry after 48 h;Immunocytochemistry and Western blot were used to investigate the Foxml expression in HNE-1 cell and its changes after using different concentrations of TST. Results The proliferation inhibition rates of TST on the HNE-1 cell after 48 h in each concentration groupd ,2,4,8,12,16 μmol/L) were 8. 31%, 17. 46%, 34. 28%, 58. 68%, 87. 91% ,99. 05% ; HNE-1 cell were kept at stage G1/G0 in 1 μmol/L and 2 μmol/L group(P<0. 05), which was dose-dependent(P<0. 05) ;Foxml protein decreased significantly in HNE-1 cell with TST in 48 h by immunocytochem-istry and Western blot test(P<0. 05). Conclusion TST inhibits the prolife ration and Foxml expression in HNE-1 cell lines, and the reason may be that the TST could decrease the Foxml expression in tumor cells.
关键词
硫链丝菌肽/Foxm1/HNE-1Key words
thiostrepton/Foxml/ HNE-1引用本文复制引用
林力,邓碧,寿铸,梁佳,陈鸿雁..硫链丝菌肽对人鼻咽癌HNE-1细胞生长及Foxm1表达的影响[J].重庆医学,2012,41(31):3283-3285,封3,4.
