重庆医学2012，Vol.41Issue(32)：3401-3403,3407,封3,5.DOI:10.3969/j.issn.1671-8348.2012.32.019
他克莫司对肾小管上皮细胞转分化早期CTGF、VEGF表达的影响
Effects of Tacrolimus on transdifferentiation and CTGF,VEGF early expression of human renal tubular epithelial cells
摘要
Abstract
Objective To explore different doses of tacrolimus(FK506) to renal tubular epithelial cells(HKC) transdifferentiation and its early action on connective tissue growth factor(CTGF) ,vascular endothelialgrowth factor (VKGF) expressing change. Methods The human kidney cells(HKC) were cultured for 48 hours in different conditions: (1)Serum free as control, (2)Treated with TGF-β1(8 ng/mL) ;(3)Treated with FK506 at different concentration(10, 30 ,60 ng/mL) ;(4)Treated with FK506 at different concentration(10 ,30,60 ng/mL) plus TGF-β1(ng/mL). The expression of crSMA was assessed with RT-PCR,The expression of CTGF,VEGF was assessed with RT-PCR and immunohistochemistry after 48 hours. Results Compared with the control group, The expression of CTGF, VEGF and crSMA mRNA was markedly increased in HCK cultured with TGF-βl group(P<0. 05) , which were suppressed significantly in HKC cultured with TGF-fSl plus FK506 group(P<0. 05). Conclusion The inhibition of FK506 on transdifferentiation of tubular epithelial cells may be related to the down-regulation of VEGF,CTGF.
关键词
他克莫司/肾小管/细胞转分化/血管内皮生长因子类/结缔组织生长因子Key words
tacrolimus/ kidney tubules/ cell transdifferentiation/ vascular endothelial growth factors/ connective tissue growth factor引用本文复制引用
闵亚丽,赵冬慧,于黔..他克莫司对肾小管上皮细胞转分化早期CTGF、VEGF表达的影响[J].重庆医学,2012,41(32):3401-3403,3407,封3,5.
