重庆医学2012，Vol.41Issue(33)：3471-3472,3475,封2,封3,5.DOI:10.3969/j.issn.1671-8348.2012.33.003
GFP标记成年大鼠表皮神经嵴干细胞的分离培养与形态学特征
Culture and morphologic characteristics of epidermal neural crest stem cell isolated from GFP adult rat
摘要
Abstract
Objective To establish methods for isolating and culturing the epidermal neural crest stem cell (EPI NCSC) from GFP adult rat,and to lay the foundation for tracking transplanted cell of spinal cord injury and repair. Methods We isolated whisk er hair follicle,and took out of bulges from collagen capsule,setting several bulges on pre incubate collagen coated culture plates. Removed bulges,4 days after onset of EPI NCSC emigration from the bulge explants,and enrichment cultured the migratory cells. The EPI NCSC were identified by the immunocytochemistry of SOX10 and Nestin,and its purity was calculated according to the positive rate. Results The EPI NCSC emigrated from the blunge explants on collagen substratum,showed morphological types of round and spindle with strong green fluorescence. More than 99% cultured cells were identified to be EPI NCSC,which expressed SOX10 and Nestin. Conclusion This method is simple. The high purity of GFP EPI NCSC can be harvested to be used as an effec tive tool cells,and widely used for researching on the spinal cord injury repairing.关键词
GFP大鼠/表皮神经嵴干细胞/细胞培养技术/免疫细胞化学Key words
GFP rat/epidermal neural crest stem cell/cell culture techniques/immunocytochemistry引用本文复制引用
张洁元,陈立朝,段朝霞,李兵仓..GFP标记成年大鼠表皮神经嵴干细胞的分离培养与形态学特征[J].重庆医学,2012,41(33):3471-3472,3475,封2,封3,5.基金项目
创伤、烧伤与复合伤国家重点实验室Ⅰ类研究课题(SKLZZ201003) （SKLZZ201003）
国家重点基础研究发展计划资助项目(973计划,2012CB518106). （973计划,2012CB518106）
