结直肠肛门外科2012，Vol.18Issue(4)：230-233,4.
牛蒡子苷元对结肠癌细胞SW480凋亡的影响及其机制
Effects of Arctigenin on the Apoptosis of Colorectal cancer and Its Mechanisms
张和平 1王敬敏2
作者信息
- 1. 焦作市中医院,河南焦作,454000
- 2. 焦作市人民医院,河南焦作,454000
- 折叠
摘要
Abstract
Objective Colorectal cancer cells SW480 were used as the target to investigate the inhibitory effects of arctigenin on the apoptosis of the Colorectal cancer cells and its primary mechanisms. Methods (1) SW480 cells were taken as the target, The apoptosis of SW480 cells were measured by agarose gel electro phoresis and flow cytometry. (2)The mechanisms of d anti-apoptotic effects of arctigenin on the SW480 cells were studied with RT-PCR assay. Results (1)Observation of agarose gel electrophoresis revealed that there were obvious ladder like collapse of sw480 cells after treated with 20mg/L arctigenin, while it was not found in the blank control group.(2)Results of flow cytometry displayed that the apoptosis number of sw480 cells was up-regulated in the cells induced with 20mg/L arctigenin{ P <0. 01). Results of flow cytometry displayed that the apoptosis number of sw480 cells was up-regulated in the cells induced with 20 mg/L arctigenin{ P <0. 01). ?Results of RT-PCR assay illustrated that expression of anti-apoptotic genes bcl-2 and IAP-1 were down-regulated, and Smac and bax were up-regulated, while bcl-XL was unchanged. Conclusion (1)This study provides a preliminary proof of arctigenin's inhibition effect on apoptosis of sw480 cells. ?This study provides a preliminary proof that the up-regulation of Smac and bax and down-regulation of bcl-2 and IAP-1 may have relation with the apoptosis inhibition of arctigenin on sw480 cells.关键词
结直肠癌/牛蒡子苷元/凋亡Key words
Colorectal cancer/Arctigenin/Apoptosis分类
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张和平,王敬敏..牛蒡子苷元对结肠癌细胞SW480凋亡的影响及其机制[J].结直肠肛门外科,2012,18(4):230-233,4.
