大豆科学2012，Vol.31Issue(5)：718-724,7.
野生大豆胁迫应答LRR类受体蛋白激酶基因的克隆及其表达特性分析
Isolation and Expression Pattern Analysis of LRR Kinase Gene Response to Osmotic Stress from Glycine soja
摘要
Abstract
One EST induced by cold represents a leueine-rich repeat receptor-like kinase(LRR-RLK)gene was identified according to the gene expression profiles of Glycine soja leaves under osmotic stresses previously constructed in our laboratory. The full-length cDNA was successfully retrieved by RACE, which was 2 264 bp in length and contains a complete ORF encoding 714 amino acids. The bioinformatics analysis showed that,the GsLRPK protein had an N-terminal signal sequence( residues 1 to 22 ) , extracellular leueine-rich repeats, a single transmembrane region, and a cytoplasmic catalytic domain, considered crucial for kinase activity. What' s more,semi-quantity PCR result showed that the GsLRPK was regulated by different stresses treatment,such as drought,ABA,4℃ cold and high salinity,and could function in the signaling of osmotic stresses.关键词
野生大豆/LRR-RLK/渗透胁迫Key words
Glycine soja/ LRR-RLK/ Osmotic stress分类
农业科技引用本文复制引用
杨靓,李翔宇,高鹏,陈庆园,郭玉双..野生大豆胁迫应答LRR类受体蛋白激酶基因的克隆及其表达特性分析[J].大豆科学,2012,31(5):718-724,7.基金项目
福建省自然科学基金项目(2011J05051) （2011J05051）
福建省教育厅科技项目(JA11080). （JA11080）
