大豆科学2012，Vol.31Issue(5)：725-730,6.
IBA和6-BA浓度配比对诱导大豆下胚轴不定芽的影响
Effect of IBA and 6-BA Ratio on Adventitious Shoot Induction from Hypocotyl in Soybean
摘要
Abstract
Hypocotyl from soybean Heinong 44 was used as the explants to investigate the effects of IRA and 6-BA and the concentration ratio on the adventitious shoot induction and elongation. The higher induction rate of adventitious shoots from hy-pocotyls was observed in the induction MS medium without 6-BA while the seeds germinated on MS medium + 1.0 mg · L-1 6-BA. Induction rate and number of adventitious shoots reached 96. 69% and 2. 31 ( shoot length≥0. 5 cm) respectively cultured for 14 days on induction MS medium with addition of 0. 1 mg·L-1 IBA. Shoot induction medium with higher concentration of 6-BA and IBA significantly reduced the induction and elongation of adventitious shoots from hypocotyl of soybean.关键词
大豆/下胚轴/不定芽/IBA/6-BAKey words
Soybean/Hypocotyl/Adventitious shoot/IBA/6-BA分类
农业科技引用本文复制引用
郭秋云,王萍,刘兆普..IBA和6-BA浓度配比对诱导大豆下胚轴不定芽的影响[J].大豆科学,2012,31(5):725-730,6.基金项目
国家转基因生物新品种培育重大专项(2009ZX08010-013B) （2009ZX08010-013B）
中央财政支持地方高校发展专项资金资助(CXTD07). （CXTD07）
