大豆科学2012，Vol.31Issue(5)：808-812,5.
转基因大豆BPS-CV127-9 PCR定量检测研究
Quantitative Detection of Genetically Modified Soybean BPS-CV127-9
摘要
Abstract
A quantitative method to detect the transgenic soybean BPS-CV-127-9 by using real-time PCR technique based on fluorescence dye SYBR Green I was investigated in this study. The endogenous lectin gene and the 5' flanking sequences of BPS-CV-127-9 were amplified through the specific primers,and the transgenic content was then calculated according to the standard curve equation. Meanwhile the specificity of PCR amplification was analyzed by corresponding melting curves. The results showed that the standard curves of lectin and the 5' flanking sequences of BPS-CV-127-9 genes have good linear relationship, and their R values were 0. 999 and 0. 998, respectively. The coefficient of variance was 1. 50%-18. 51% and standard deviation was 0.02-0.07. Four mixed samples with genetically modified contents of BPS-CV-127-9 was 0.05% ,0.1% ,0.5% and 1% respectively were detected,and the detection results agreed well with actual value. In conclusion,this method was fast, sensitive, simple, accurate, specific and of high throughput, and could be used to detect transgenic soybean BPS-CV-127-9 quantificationally.关键词
品系特异性/实时荧光定量PCR/转基因大豆Key words
Event-special/Real-time PCR/Genetically modified soybean分类
农业科技引用本文复制引用
李宁,赵蕾,孙红炜,李凡,杨淑珂,路兴波..转基因大豆BPS-CV127-9 PCR定量检测研究[J].大豆科学,2012,31(5):808-812,5.基金项目
国家转基因重大专项(2008ZX08012-001). （2008ZX08012-001）
