动物营养学报2012，Vol.24Issue(9)：1832-1838,7.DOI:10.3969/j.issn.1006-267x.2012.09.030
一株乳酸菌GF103的分离鉴定及体外益生效果评价
Isolation and Identification of Lactobacillus sp.GF103 and Assess It as Potential Probiotics in Vitro
摘要
Abstract
This study was aimed to identify strain properties of Lactobacillus sp. GF103 and assess it as potential probiotics in vitro. Lactobacillus sp. GF103 , which could inhibit the growth of pathogenic bacteria Esche-richia coli and Staphylococcus aureus, was isolated from soil nearby Beijing Daxing breeding pig farm. And it was identified as Lactobacillus plantarum by 16S rRNA sequence analysis. Learned by the assessment of potential prebiotics in vitro, a large amount of the strain GF103 survived under the condition of pH =3. The strain GF103 had the ability to tolerance of 0.3% bile salt. The viable counts of strain GF103 did not change after 0.5 h (P> 0.05) in simulated gastric fluid and decreased 1 log value after3 h (P<0.01). The viable counts of strain GF103 did not decrease in simulated small intestinal fluid after 3 h (P >0.05) . These results suggest that the strain GF103 has probiotic properties and the effects on its application in animals as feed additives can be further studied. [Chinese Journal ofAnimal Nutrition, 2012, 24(9) : 1832-1838]关键词
乳酸菌/16S rRNA基因/鉴定/耐受性Key words
Lactobacillus sp. / 16S rRNA gene/ identification/ tolerance分类
农业科技引用本文复制引用
董晓丽,张乃锋,周盟,屠焰,刁其玉,聂明非..一株乳酸菌GF103的分离鉴定及体外益生效果评价[J].动物营养学报,2012,24(9):1832-1838,7.基金项目
农业部引进国际先进农业科学技术重点项目"低碳氮排放的饲料高效利用技术引进与研发"(2011-G7) （2011-G7）
现代农业产业技术体系北京市创新团队专项资金 （）
