动物营养学报2012，Vol.24Issue(10)：1976-1982,7.DOI:10.3969/j.issn.1006-267x.2012.10.017
饲粮ω-6/ω-3多不饱和脂肪酸比例对鹅肝脏过氧化脂质和肝细胞超微结构的影响
Dietary ω-6/ω-3 Polyunsaturated Fatty Acid Ratio Affects Lipid Peroxide and Ultrastructure in Geese Liver
摘要
Abstract
The objective of this study was to investigate the effects of dietary ω-6/ω-3 polyunsaturated fatty acid (PUFA) ratios on lipid peroxide and cellular ultrastructure in geese liver. A total of 160 healthy Yangzhou geese with similar weight (0. 407 ±0. 023) kg, aged 21 days, were randomly divided into 4 groups with 4 replicates per group and 10 geese per replicate. They were fed the diets with ω-6/ω-3 PUFA ratios of 12:1, 9:1, 6:1, and 3:1, respectively. The liver was separated and lipid peroxides were tested at 42, 56, and 70 days of age. The results showed as follows: the contents of malondialdehyde (MDA) and nitric oxide (NO) in liver showed an ascendant tendency with age increasing, and the highest was at 70 days of age. The increasing ranges of 12:1 and 9:1 groups were higher than these of 6:1 and 3:1 groups. It was also observed that the contents of MDA and NO showed a declined tendency with the decreasing of ω-6/ω-3 PUFA ratios, and the lowest was in 3:1 group regardless the detected ages. The cellular ultrastructure observation showed that dietary ω-6/ω-3 PUFA ratios in 12:1 and9:1 groups decreased the number of hepatocyte glycogen pellets, and swelled mitochondria; but there was no significant difference in the average area of mitochondria among all groups (P>0.05). In conclusion, dietary w-6/ω-3 PUFA ratio affects the hepatic antioxidant function in growing Yangzhou geese, and dietary lower ω-6/ω-3 PUFA ratio, such as 3:1 and 6:1, can decrease hepatocytes damage of lipid peroxide. [ Chinese Journal of Animal Nutrition, 2012, 24 (10) : 1976-1982 ]关键词
ω-6/ω-3 PUFA比例/肝脏/抗氧化功能/超微结构Key words
ω-6/ω-3 PUFA ratio/ liver/ antioxidant function/ ultrastructure分类
农业科技引用本文复制引用
丁洛阳,蔡缪荧,王梦芝,喻礼怀..饲粮ω-6/ω-3多不饱和脂肪酸比例对鹅肝脏过氧化脂质和肝细胞超微结构的影响[J].动物营养学报,2012,24(10):1976-1982,7.基金项目
江苏省自然基金基础研究项目(SBK201121885) （SBK201121885）
扬州大学2011大学生科技创新基金项目(B11098) （B11098）
