动物营养学报2012，Vol.24Issue(10)：2036-2043,8.DOI:10.3969/j.issn.1006-267x.2012.10.025
饲粮NFC/NDF对奶山羊瘤胃上皮细胞胰岛素样生长因子Ⅰ及其受体的基因表达的影响
Dietary NFC/NDF Affects Expressions of IGF-Ⅰ and IGF-Ⅰ R Genes in the Ruminal Epithelium of Dairy Goats
摘要
Abstract
The objective of this study was to investigate the changes of expressions of insulin-like growth factor I (IGF- I ) and insulin-like growth factor I receptor (IGF- \ R) genes in the ruminal epithelium of dairy goats in the process of inducing subacute rumen acidosis ( SARA) under the condition of different dietary ratios of non-fiber carbohydrate and neutral detergent fiber (NFC/NDF), This study was designed for four experimental periods with twelve lactating Guanzhong dairy goats, and 15 days in each period. Goats were fed 4 diets with different NFC/NDF (1. 02 , 1. 24, 1. 63 and 2. 58 , respectively). The induction of SARA was conducted by the gradual increase of dietary NFC/NDF, and expressions of IGF-1 and IGF- I R genes were quantitatively analyzed by quantitative real-time PCR (qRT-PCR). The results showed as follows: expressions of IGF- I and IGF- I R genes were both increased with the gradual increase of dietary NFC/NDF. The expression of IGF- I gene in periods II , E and FV was 1. 70 , 2.71 and 9.61 times of that in period I , respectively , and the expression of IGF- I R gene in periods II , 1 and IV was 1.88, 3. 09 and 10.19 times of that in period I , respectively. Compared with period I , expressions of IGF- I and IGF- I R genes significantly were increased when NFC/NDF reache 2.58(F<0.01). In conclusion, induction of SARA using the method of increasing dietary NFC/NDF can significantly increase expressions of IGF- I and IGF- I R genes, and they are increased sharply after SARA occurs. [ Chinese Journal of Animal Nutrition, 2012, 24(10) :2036-2043]关键词
亚急性瘤胃酸中毒/瘤胃上皮细胞/实时定量PCR/胰岛素样生长因子Ⅰ/胰岛素样生长因子Ⅰ受体Key words
subacute rumen acidosis/ ruminal epithelium/ quantitative real-time PCR/ insulin-like growth factor I / insulin-like growth factor I receptor分类
农业科技引用本文复制引用
周向丽,刘大程,孙鸽,高民,胡红莲..饲粮NFC/NDF对奶山羊瘤胃上皮细胞胰岛素样生长因子Ⅰ及其受体的基因表达的影响[J].动物营养学报,2012,24(10):2036-2043,8.基金项目
国家自然科学基金项目(30960252) （30960252）
现代农业产业技术体系建设专项资金资助 （）
