南方医科大学学报2012，Vol.32Issue(10)：1440-1444,5.DOI:10.3969/j.issn.1673-4254.2012.10.012
HSV-2 LAT过表达vero细胞中microRNA的表达谱变化
Changes of microRNA expression profiles in Vero cells induced by HSV-2 LAT overexpression
摘要
Abstract
Objective To investigate the changes in the microRNA expression profile of Vero cells induced by HSV-2 LAT overexpression. Methods The full-length open reading frame of HSV-2 LAT was synthesized and cloned into pRetroQ-AcGFP1-C1 vector, and the recombinant retrovirus expressing HSV-2 LAT was packaged. Using a microRNA microarray, the microRNA expression profile changes in Vero cells were analyzed after infection with the recombinant retrovirus. Results In Vero cells infected with the recombinant retrovirus for stable HSV-2 LAT overexpression, 5 microRNAs (hsa-miR-23a*, kshv-miR-K12-3, hsa-miR-943, hsa-miR-634, and hsa-miR-1270) were up-regulated and 5 (hsa-miR-181a-2*, hsa-miR-450b-5p, hsa-miR-31, hsa-miR-24, and kshv-miR-K12-12*) were down-regulated. Conclusion The expression of HSV-2 LAT can induce changes in microRNA expression profile in Vero cells.关键词
疱疹病毒Ⅱ型/LAT/microRNA/基因芯片/逆转录病毒Key words
herpes simplex virus type 2/ LAT/ microRNA/ microarray/ retrovirus分类
医药卫生引用本文复制引用
王颖,樊建勇,杨慧兰,陈剑云..HSV-2 LAT过表达vero细胞中microRNA的表达谱变化[J].南方医科大学学报,2012,32(10):1440-1444,5.基金项目
国家自然科学基金(81071401) （81071401）
广东省科技计划(2009B080701091) （2009B080701091）
