福建农业学报2012，Vol.27Issue(8)：787-791,5.
鳗鲡疱疹病毒ORF95基因的克隆及其在大肠杆菌中的表达
Prokaryotic Expression of Anguillae herpesvirus ORF95 Protein
摘要
Abstract
Anguillae herpesvirus (AngHV) is one of the major viral pathogen of freshwater eels . In this study, the open reading frame of AngHV-FJ ORF95 gene was synthesized according to the gene sequence of AngHV-1 provided by GenBank: (NC-013668), and then cloned into the pMD19-T vector. After restriction digestion and sequence verification, the ORF95 gene was inserted into the prokaryotic expression vector pET-32a to construct the recombinant expression plasmid 32a-ORF95. The plasmid was then transformed into E. colt BL21 (DE3) strain to induce expression of ORF95 protein. The soluble ORF95 protein can be highly expressed under the induction of IPTG (0. 1 mmol · L-1 ) at 23℃ for 14h, and then purified by Ni-NTA His. Binding affinity purification. The protein can be used further to understand the characteristic of ORF95 protein and the AngHV prevention.关键词
鳗鲡疱疹病毒/ORF95/基因克隆/原核表达Key words
Anguillae herpesvirus/ ORF95/ gene cloning/ prokaryotic expression分类
农业科技引用本文复制引用
李友娟,葛均青,宋铁英,林天龙..鳗鲡疱疹病毒ORF95基因的克隆及其在大肠杆菌中的表达[J].福建农业学报,2012,27(8):787-791,5.基金项目
国家自然科学基金项目(31101933) （31101933）
福建省科技计划项目——省属公益类科研院所基本科研专项(2009R10035-6) （2009R10035-6）
福建省科技平台建设项目(2009N2003) （2009N2003）
