福建农业学报2012，Vol.27Issue(8)：792-795,4.
福建省猪副猪嗜血杆菌病血清学调查及菌株ompP5基因序列分析
Serological Survey and ompP5 Gene Analysing of Haemophilus Parasuis Derived from Fujian Province
摘要
Abstract
Hps antibody of 378 serum sample from 23 pig farm in 8 city of Fujian Province was detected by the indirect ELISA reagent kit; And Hps was separated and identified by use of TSA plate with removal of fine horse blood from the collected disease materials of swine suspected infecting Hps; And the serotype of separated strains was classified by the Agar diffusion tests And a pair of specific primers was synthesized by referring to ompP5 gene of HS0165 strain signed in GenBank, and the ompP5 gene was amplified using Hps DNA extracted as a template by PCR method, cloned, sequenced and blasted. The results showed that the positive rate in 23 field from Fujian Province was to 100%, and the positive rate of the herd was to 36. 78%; 52 isolates from the swine of suspected infecting Hps had been separated and identified, and were regarded as serotype 2, 4, 5, 13 and non—serotype respectively, in addition, the serotype 13 was the most; Compared the GenBank, the homology of ompP5 of 3 isolates are respectively up to 93%.关键词
福建省/副猪嗜血杆菌/血清学/血清型/外膜蛋白P5基因Key words
Fujian/ haemophilus parasuis/ serology/ serotype/ ompP5 gene分类
农业科技引用本文复制引用
车勇良,王隆柏,陈如敬,江斌,吴学敏,刘玉涛,庄向生,周伦江..福建省猪副猪嗜血杆菌病血清学调查及菌株ompP5基因序列分析[J].福建农业学报,2012,27(8):792-795,4.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2010R1025-2) （2010R1025-2）
国家公益性行业(农业)科研专项(NYHYZX07-034) （农业）
福建省农业科学院"双百"行动项目(sbmx1202) （sbmx1202）
