福建农业学报2012，Vol.27Issue(9)：913-918,6.
水稻日本晴IPA1 cDNA的克隆及植物过表达载体的构建
Cloning and Construct of Over-expression Vector of IPA1 cDNA from Nipponbare
摘要
Abstract
A cDNA encoding IPA1 was isolated from Nipponbare by RT-PCR, sequencing results showed that the sequence consists of 1 257 bp with a complete CDS and encodes a protein of 418 amino acids. In addition, the prediction results indicated that molecular weight of target protein was 42. 51 kD and theoretical isoelectric point was 9. 37. The plant expression vector was constructed by ligating the cDNA fragment into expression vector pHI, then the recombinant plasmid pHl-7PAl was confirmed by restriction enzyme digestion analysis with SmaI、SacI, and further verified by DNA sequencing. Vector plasmid was transformed into Agrobacterium tumefaciens, which will lay foundation for rice genetic transformation.关键词
水稻/IPA1/cDNA克隆/载体构建Key words
rice/IPA1 /cDNA cloning/ vector construction分类
农业科技引用本文复制引用
连玲,何炜,蔡秋华,许惠滨,朱永生,张建福,谢华安..水稻日本晴IPA1 cDNA的克隆及植物过表达载体的构建[J].福建农业学报,2012,27(9):913-918,6.基金项目
国家转基因作物新品种培育重大专项(2011ZX08001-004) （2011ZX08001-004）
福建省自然科学基金项目(2012J01109) （2012J01109）
福建省农业科学院青年基金项目(2011QA-3) （2011QA-3）
福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y04) （STIF-Y04）
