福建农业学报2012，Vol.27Issue(9)：919-923,5.
3'RACE法扩增谷氨酰胺合成酶基因及其生物信息学分析
Glutamine Synthetase Gene Amplification by 3'RACE and Bioinformatics Analysis
摘要
Abstract
On the basis of GS mRNA and specific primer, Using 3'RACE PCR amplified the gene sequence and analyzed some bioinformatics information of the sequence in NCBI web. GS DNA fragment was about 1. 0 kb. The sequence blast showed that the homology of fragment with GS gene of Asp. niger in GenbBank was more than 97 % and with other Asp were more than 50%. Open reading frame analyzed that it included 2 exon, one of which had 100% homology with GS of Asp. niger.关键词
谷氨酰胺合成酶基因/3'RACE/生物信息学Key words
glutamine synthetase gene/ 3'RACE/ bioinformatics分类
生物科学引用本文复制引用
张志灯,罗土炎,饶秋华,曹治云,郑腾..3'RACE法扩增谷氨酰胺合成酶基因及其生物信息学分析[J].福建农业学报,2012,27(9):919-923,5.基金项目
福建省科技计划项目(2000Z030) （2000Z030）
