福建农业学报2012，Vol.27Issue(9)：924-930,7.
粳稻云引中一个受稻瘟病菌诱导的抗逆候选基因的克隆及表达分析
Functional Analysis of a Candidate Gene in Relation to Stress Resistance Induced with Magnaporthe grisea from Japonica Variety Yunyin
摘要
Abstract
Based on gene chip of japonica rice cv. Yunyin induced with Magnaporthe grisea , one of the candidate genes up-regulated remarkably by Sichuan43 s isolation was cloned. Sequence and phylogenetic tree analyses suggest that the gene is closest relative to Oryza sativa L. AF529237. 2 and Tritieum aestivum X51608. 1 with 99. 0% and 88. 0% identity, and may relate to phosphoribulo kinase which is a critical enzyme in calvin cycle. Subsequently, the overexpression, RNAi expression and transient expression vector were constructed to obtain corresponsive transgenic lines, respectively. All these transgenic lines generated were used to assess the function of the gene in the stress response.关键词
粳稻云引/稻瘟病菌/抗逆候选基因/功能分析Key words
Japonica rice Yunyin/ Magnaporthe grisea/ candidate gene/ functional analysis分类
农业科技引用本文复制引用
何炜,连玲,熊海清,蔡秋华,朱永生,谢鸿光,王颖姮,张建福,谢华安..粳稻云引中一个受稻瘟病菌诱导的抗逆候选基因的克隆及表达分析[J].福建农业学报,2012,27(9):924-930,7.基金项目
国家"973"计划项目(2012CB723003) （2012CB723003）
国家"863"计划项目(2011AA10A101) （2011AA10A101）
福建省杰出青年基金项目(2009J06011) （2009J06011）
福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y04) （STIF-Y04）
