福建农业学报2012，Vol.27Issue(9)：945-950,6.
雏番鸭胰腺型鸭1型甲肝病毒分离鉴定及VP1基因分析
Identification and Sequence Analysis of Duck Hepatitis A Virus Type 1 Isolated from Muscovy Duckling with Pancreatitis
摘要
Abstract
Samples came from Muscovy ducklings with yellowed or hemorrhagic pancreatitis were positive for duck hepatitis A virus-1 (DHAV-1) by RT-PCR. A virus strain (named as MPZJ1206) was abtained from Virus-containing allantoic fluid after inoculation of embryonated Muscovy duck eggs with DHAV-1 -positive samples. The isolate shows no hemagglutinating activity with chickens, ducks or sheep red blood cells at different temperature. Plaque purified virus was specifically neutralized by anti-DHAV-1 hyperimmune serum. Seven-day-old Muscovy ducklings inoculated with the virus showed a mortality rate of 38.5%, and yielded the same pathological characteristics as those of clinical onset duck had. The virus strain also recovered from the pancreas of Muscovy duckling died in the trials. A 714-bp fregment gene was abtained by using a set of primers aimed at VP1 ORF gene, and shared 99.1% nucleotide sequence identity with that of classical DHAV-1 strain Du/CH/LGD/111239. Phylogenetic analysis showed that MPZJ1206 strain lay in the viral lineage of DHAV-1. The above results indicate that pancreatitis in Muscovy ducklings was caused due to the infection of duck hepatitis A virus type 1, and this pathotype is distincted from hemorrhagic hepatitis caused by classical duck hepatitis A virus, so it advised that the virus isolated in this study could be named as pancreatitis-type DHAV-1.关键词
鸭甲肝病毒/胰腺型/雏番鸭/VP1衣壳蛋白Key words
duck hepatitis A virus/ pancreatitis/ muscovy duckling/ VP1 protein分类
农业科技引用本文复制引用
傅光华,林建生,林芳,陈红梅,黄瑜,施少华,彭春香,江斌,程龙飞,万春和,傅秋玲..雏番鸭胰腺型鸭1型甲肝病毒分离鉴定及VP1基因分析[J].福建农业学报,2012,27(9):945-950,6.基金项目
现代农业产业技术体系建设专项资金(CARS-43) （CARS-43）
福建省自然科学基金(2011J01113) （2011J01113）
福建省种业创新专项(2011FJZY-9) （2011FJZY-9）
福建省农业科学院科技下乡"双百"行动项目(SBMX1224) （SBMX1224）
