福建师范大学学报（自然科学版）2012，Vol.28Issue(6)：100-105,6.
ScINO1基因克隆及酵母多基因多拷贝整合表达载体的构建
Cloning of ScINO1 Gene and Construction of Yeast Multi-copy Integration Expression Vector
摘要
Abstract
The inositol-1-phosphate synthase encoding gene (ScINOl) was amplified from the genomic DNA of Saccharomyces cerevisiae S288c by PCR. The length of ScINOl gene was 1 602 bp. The Blast results showed that ScINOl gene sequence was consistent with sequence of S. cerevisiae S288c in GenBank. The digested products of PGK, CYCl and a rDNA fragment were inserted into S. cerevisiae shuttle plasmid PUG6 to generate rDNA mediated multi-copy integrated expression vectors pURH . Then, to obtain multi-copy integrated expression vectors pURIH, the ScINOl gene was inserted into pURH. The yeast multi-copy integration expression vector was further verified through double restriction enzyme digestion. The construction of yeast multi-copy integration expression vector was provided with basic material for further study in genetic engineering of synthesis inositol and high ethanol tolerance in yeast.关键词
ScINO1基因/多拷贝整合/载体构建/酿酒酵母Key words
ScINO1 gene/ multi-copy integration/ vector construction/ Saccharomyces cerevisiae分类
农业科技引用本文复制引用
黄贞杰,陈玲,张积森,叶冰莹,陈由强,陈如凯..ScINO1基因克隆及酵母多基因多拷贝整合表达载体的构建[J].福建师范大学学报（自然科学版）,2012,28(6):100-105,6.基金项目
现代农业产业技术体系建设专项资金资助项目(CARS-20-4-4) （CARS-20-4-4）
