分析测试学报2012，Vol.31Issue(9)：1070-1074,5.DOI:10.3969/j.issn.1004-4957.2012.09.008
基于两相预柱的在线二维分离系统用于人肝组织中磷酸肽的规模化鉴定
Online Multidimensional Separation System Based on the Biphasic Trap Column for Large Scale Phosphorylation Analysis of Human Liver
摘要
Abstract
A fully automated online multidimensional separation system, which is based on a reversed phase-strong cation exchange(RP - SCX) biphasic trap column, was developed for the large scale phosphorylation analysis of human liver. Compared with the conventional online SCX - RP system and off - line mode, the biphasic column can reduce the sample loss and improve the detection sensitivity. This system was applied in the large scale phosphorylation analysis of human liver and a total of 3 082 unique phosphopeptides, including 3 056 unique phosphorylation sites corresponding to 1 332 phosphoproteins were identified under the FDR ＜ 1% . The molecular function and motif analysis of phosphoproteins has revealed the important role of phosphorylation in the liver physiological functions.关键词
在线多维色谱/磷酸肽/蛋白质组学/人肝Key words
online multidimensional separation/ phosphopeptides/ proteomics/ human liver分类
化学化工引用本文复制引用
宋春侠,王方军,程凯,边阳阳,叶明亮,邹汉法..基于两相预柱的在线二维分离系统用于人肝组织中磷酸肽的规模化鉴定[J].分析测试学报,2012,31(9):1070-1074,5.基金项目
中国科学院科研装备研制项目(P020090520) （P020090520）
国家自然科学基金创新群体项目(21021004) （21021004）
