分析测试学报2012，Vol.31Issue(10)：1236-1241,6.DOI:10.3969/j.issn.1004-4957.2012.10.004
基于明胶-碳纳米管的辣根过氧化物酶修饰电极在离子液体中的直接电化学
Direct Electrochemistry of a GCE Modified with HRP/Gel-MWCNTs in Ionic Liquid [BMIM] BF4
摘要
Abstract
A novel hydrogen peroxide biosensor was constructed based on horseradish peroxidase (HRP) immobilized onto glass carbon electrode (GCE) , which was modified with the nanocomposite of gelatin and multi-walled carbon nanotubes ( MWCNTs ). The electrochemistry and electrocatalysis of the modified biosensor were investigated. A pair of well-defined and nearly symmetrical redox peaks with a formal potential of -0.356 Y(vs. SCE) in PBS were observed, which attribute to the direct electron transfer between the entrapped HRP and the electrode. The redox peak current was proportional to scan rate in the range of 20 -180 mV/s, indicating that the process was affected by e-lectronic transfer electrode rate control surface of mass transfer process. The electrochemical characteristics of the modified electrode were investigated by cyclic voltammetry. The effects of applied potential , solution pH and interferences on the biosensor were discussed. The results demonstrated that HRP could display an excellent electrocatalytic response to the reduction of hydrogen peroxide. Furthermore, the biosensor exhibited a more sensitive catalytic activity on H2O2 in a hydrophilic ionic liquid, l-butyl-3-methylimidazolium tetrafluoroborate ( [ BMIM ] BF4 ) , than that in PBS buffer. A wide linear response to H2O2 in the range of 2. 0 × 10 -0. 13 mol/L was obtained with a detection limit ( S/N = 3 ) of 2. 3 ×10 -8 mol/L. The modified electrode exhibited the advantages of good stability , wide linear range and lower detection limit as well as a certain anti-interference ability.关键词
碳纳米管/明胶/辣根过氧化物酶/离子液体/过氧化氢Key words
carbon nanotubes/ gelatin/ horseradish peroxidase/ ionic liquid/ hydrogen peroxide分类
化学化工引用本文复制引用
姚慧,王燕,董元,孙迪,张严化..基于明胶-碳纳米管的辣根过氧化物酶修饰电极在离子液体中的直接电化学[J].分析测试学报,2012,31(10):1236-1241,6.基金项目
国家自然科学基金资助项目(21071100) （21071100）
沈阳化工大学博士启动基金资助项目(20063209) （20063209）
