分析测试学报2012，Vol.31Issue(10)：1282-1287,6.DOI:10.3969/j.issn.1004-4957.2012.10.012
薄层荧光扫描法测定喜树果中喜树碱及10-羟基喜树碱
Determination of Camptothecine and 10-Hydroxycamptothecine in Common Camptotheca Fruit by TLC-Fluorescence Scanning Method
摘要
Abstract
A thin layer chromatography ( TLC) - fluorescence scanning method was established for the determination of camptothecine ( CPT) and 10-hydroxycamptothecine-( 10-HCPT) in Chinese herbal medicine Xishuguo ( Common camptotheca fruit, CCF). Methanol was used as solvent to extract CPT and 10-HCPT in CCF sample. The separation was carried out on silica gel H plate using chloroform -acetone (7 : 3 ) as developing agent. The determination was accomplished by fluorescence linear scanning with excitation wavelength of 350 nm. In the quality range of 0. 010 5 -0. 063 0μg, there is a linear relationship between integral fluorescence intensity and quality of CPT. The regression e-quation was A =474. 0 + 159 881. 2 m(μg) with a correlation coefficient of 0. 998. The spiked recovery of CPT was 99% . In the quality range of 0. 003 3-0. 033 0 μg, a linear relationship between integral fluorescence intensity and quality of 10-HCPT was observed, and the regression equation was A =51. 3 +59 486. 2 m( μg) with a correlation coefficient 0. 999. The spiked recovery of 10-HCPT was 96% . This method was simple and repeatable. Using this method, the contents of CPT and 10-HCPT in a CCF sample were determined to be 0. 122% and 0. 013% , respectively.关键词
中药/喜树果/喜树碱/10-羟基喜树碱/薄层荧光扫描Key words
traditional Chinese medicine/ common camptotheca fruit/ camptothecine/ 10-hydroxycamptothecine/ TLC - fluorescence scanning分类
化学化工引用本文复制引用
王立屏,魏永巨..薄层荧光扫描法测定喜树果中喜树碱及10-羟基喜树碱[J].分析测试学报,2012,31(10):1282-1287,6.基金项目
国家自然科学基金资助(20675025,20975029) （20675025,20975029）
