分析测试学报2012，Vol.31Issue(10)：1288-1293,6.DOI:10.3969/j.issn.1004-4957.2012.10.013
化妆品中5种禁用着色剂的高效液相色谱检测及质谱确证
Determination of Five Prohibited Coloring Agents in Cosmetics by HPLC and Their Verification by HPLC-MS/MS
摘要
Abstract
An HPLC method was developed for the determination of acid yellow 36, pigment red 53 : 1, pigment orange 5, sudan Ⅱ and sudan Ⅳ in cosmetics. The extraction conditions of five prohibited coloring agents in cosmetic samples, including wax, powder and liquid, were investigated. Using tetrabutylammonium hydroxide as an ion-pair reagent, the optimum chromatographic conditions of the prohibited coloring agents were investigated. The optimal conditions were as the follows: stationary phase; Hypersil C18(4. 6 mm ×250 mm, 5 μm) , mobile phase: acetonitrile(A) -0.01 mol/L tetrabutylammonium hydroxide and 0.01 mol/L citric acid(pH 8.2, B) , flow rate; 1.0 mL/ min, column temperature; 30 ℃ and detection wavelengths; 416, 484, 514 nm. The calibration curves of five prohibited coloring agents showed good linearities in the range of 0. 2 - 50 mg/L with correlation coefficients more than 0. 999 and LODs(S/Af =3) of 0. 2 -0. 5 mg/L. The five prohibited coloring agents were verified by HPLC — MS/MS. At three spiked levels, the average recoveries were between 85% and 102% , with relative standard deviations of 0. 5% - 3. 6% . The method was accurate, simple and rapid, and could be used to determine acid yellow 36, pigment red 53 : 1, pigment orange 5, sudan Ⅱ and sudan Ⅳ in cosmetics.关键词
禁用着色剂/化妆品/高效液相色谱法/高效液相色谱-质谱/质谱Key words
prohibited coloring agents/ cosmetics/ HPLC/ HPLC - MS/MS分类
化学化工引用本文复制引用
王烨,马强,白桦,王超,丁岚,孟宪双,陈云霞..化妆品中5种禁用着色剂的高效液相色谱检测及质谱确证[J].分析测试学报,2012,31(10):1288-1293,6.基金项目
质检公益性行业科研专项计划项目(200910267,201010023) （200910267,201010023）
国家标准化管理委员会计划项目(20090918-T-607) （20090918-T-607）
