分析测试学报2012，Vol.31Issue(10)：1319-1324,6.DOI:10.3969/j.issn.1004-4957.2012.10.019
高效液相色谱测定食品中的7种抗氧化剂
Determination of Seven Antioxidants in Food by High Performance Liquid Chromatography
李兴根 1乔勇升 1韩芷玲 1黄银波 1董贵军1
作者信息
- 1. 泰州市产品质量监督检验所,江苏泰州225300
- 折叠
摘要
Abstract
A method was developed for the simultaneous determination of 7 antioxidants, including butylated hydroxyanisole ( BHA ) , butylated hydroxytoluene ( BHT) , tertiary butylhydroquinone ( TB-HQ) , propyl gallate(PG) , octyl gallate(OG) , iso-amyl gallate(IAG) and dodecyl gallate(DG) , in food using high performance liquid chromatographic ( HPLC) method. The liquid oil sample was extracted with methanol. The extract was dried under N2 and redissolved with methanol. The solid sample was extracted with hexane, and then back extracted by acetonitrile repeatedly. The extract was also dried under N2 and redissolved with methanol. The separation of analytes was performed on an Eclipse XDB - C18 column (250 mm ×4. 6 mm i. d. , 5 μm) by gradient elution using methanol -1. 5% acetic acid water solution as mobile phase at a flow rate of 1. 0 mL/min. The sample was separated and detected with photodiode array detector(PDA) . The qualification analysis was performed by using retention time and UV spectrum, and the quantification analysis was based on the detection wavelength of 280 nm. Good linearities between concentrations of 7 analytes and peak area were a-chieved over the range of 1 - 100 mg/L with correlation coefficients( r2) larger than 0. 999. The limits of quantitation ( LOQs, S/N = 10) of 7 analytes ranged from 1. 0 mg/kg to 1. 5 mg/kg. The recoveries ranged from 79% to 101% , with relative standard deviations (RSDs) less than 7% . The method was successfully applied in the determination of 7 antioxidants residues in foods, such as cooking oil and nuts. The method is accurate, rapid and sensitive, and complys with the regulations for the determination of additives residues in foods.关键词
高效液相色谱/抗氧化剂/食品Key words
high performance liquid chromatography ( HPLC ) / antioxidants/ food分类
化学化工引用本文复制引用
李兴根,乔勇升,韩芷玲,黄银波,董贵军..高效液相色谱测定食品中的7种抗氧化剂[J].分析测试学报,2012,31(10):1319-1324,6.
